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negative controls nc pcdna  (Sangon Biotech)

 
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    Sangon Biotech negative controls nc pcdna
    Negative Controls Nc Pcdna, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/negative+controls+nc+pcdna/pmc12336314-56-6-12?v=Sangon+Biotech
    Average 86 stars, based on 1 article reviews
    negative controls nc pcdna - by Bioz Stars, 2026-08
    86/100 stars

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    GATA5 binds to the TMEM100 promoter and regulates TMEM100 expression. a, The binding site of GATA5 and TMEM100. mRNA (b) and protein level (c) of GATA5 were measured after GATA5 was overexpressed or silenced. mRNA (d) and protein level (e) of TMEM100 were detected after GATA5 was overexpressed or silenced. ***P < 0.001 versus sh-NC. F, TMEM100 promoter activity was evaluated by luciferase reporter assay. G, Chromatin immunoprecipitation assay was carried out tom detect the binding level of GATA5 and TMEM100 promotor. Data are expressed as mean ± SD. ***P < 0.001 versus control. ### P < 0.001 versus Oe-NC.

    Journal: Bioengineered

    Article Title: GATA binding protein 5-mediated transcriptional activation of transmembrane protein 100 suppresses cell proliferation, migration and epithelial-to-mesenchymal transition in prostate cancer DU145 cells

    doi: 10.1080/21655979.2021.2018979

    Figure Lengend Snippet: GATA5 binds to the TMEM100 promoter and regulates TMEM100 expression. a, The binding site of GATA5 and TMEM100. mRNA (b) and protein level (c) of GATA5 were measured after GATA5 was overexpressed or silenced. mRNA (d) and protein level (e) of TMEM100 were detected after GATA5 was overexpressed or silenced. ***P < 0.001 versus sh-NC. F, TMEM100 promoter activity was evaluated by luciferase reporter assay. G, Chromatin immunoprecipitation assay was carried out tom detect the binding level of GATA5 and TMEM100 promotor. Data are expressed as mean ± SD. ***P < 0.001 versus control. ### P < 0.001 versus Oe-NC.

    Article Snippet: The pc-DNA3.1 vector containing the whole length of TMEM100 (Oe-TMEM100), GATA binding protein 5 (GATA5)-specific pcDNA overexpression plasmid (Oe-GATA5) and negative control empty pcDNA (Oe-NC), short hairpin (sh) RNA targeting GATA5 (sh-GATA5-1/2) and the corresponding negative control (sh-NC) were synthesized by Shanghai GeneChem Co., Ltd.

    Techniques: Expressing, Binding Assay, Activity Assay, Luciferase, Reporter Assay, Chromatin Immunoprecipitation, Control

    Effects of GATA5 silencing on TMEM100-mediated migration, invasion and EMT of DU145 cells. Cell proliferation was identified by CCK-8 assay (a) and colony formation assay (b). C, Wound healing assay was implemented to assess cell migration. D, Transwell assay was employed to detect cell invasion. E, Protein levels of MMP2 and MMP9 were investigated by Western blot assay. F and G, EMT-related proteins was determined with detection of E-cadherin, N-cadherin, Vimentin and Snail. Data are expressed as mean ± SD. ***P < 0.001 versus Oe-NC. # P < 0.05, ## P < 0.01, ### P < 0.001 versus Oe-TMEM100+ sh-NC.

    Journal: Bioengineered

    Article Title: GATA binding protein 5-mediated transcriptional activation of transmembrane protein 100 suppresses cell proliferation, migration and epithelial-to-mesenchymal transition in prostate cancer DU145 cells

    doi: 10.1080/21655979.2021.2018979

    Figure Lengend Snippet: Effects of GATA5 silencing on TMEM100-mediated migration, invasion and EMT of DU145 cells. Cell proliferation was identified by CCK-8 assay (a) and colony formation assay (b). C, Wound healing assay was implemented to assess cell migration. D, Transwell assay was employed to detect cell invasion. E, Protein levels of MMP2 and MMP9 were investigated by Western blot assay. F and G, EMT-related proteins was determined with detection of E-cadherin, N-cadherin, Vimentin and Snail. Data are expressed as mean ± SD. ***P < 0.001 versus Oe-NC. # P < 0.05, ## P < 0.01, ### P < 0.001 versus Oe-TMEM100+ sh-NC.

    Article Snippet: The pc-DNA3.1 vector containing the whole length of TMEM100 (Oe-TMEM100), GATA binding protein 5 (GATA5)-specific pcDNA overexpression plasmid (Oe-GATA5) and negative control empty pcDNA (Oe-NC), short hairpin (sh) RNA targeting GATA5 (sh-GATA5-1/2) and the corresponding negative control (sh-NC) were synthesized by Shanghai GeneChem Co., Ltd.

    Techniques: Migration, CCK-8 Assay, Colony Assay, Wound Healing Assay, Transwell Assay, Western Blot